Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction
Joint Authors
Zhang, Zhenzhen
He, Zuping
Li, Peng
Hu, Hongliang
Tian, Ruhui
Zhang, Wei
Zhu, Yong
Guo, Xizhi
Huang, Yiran
Yang, Shi
Ma, Meng
Li, Zheng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Generation of germ cells from pluripotent stem cells in vitro could have great application for treating infertility and provides an excellent model for uncovering molecular mechanisms controlling gametogenesis.
In this study, we explored the differentiation potential of mouse induced pluripotent stem (iPS) cells towards male germ cells.
Embryoid body formation and retinoic acid/testosterone induction were applied to promote differentiation of mouse iPS cells into male germ cells in vitro.
Quantitative RT-PCR and immunoflourescence were performed to characterize the iPS cell differentiation process, and notably there were different temporal expression profiles of male germ cell-associated genes.
The expression of proteins, including MVH, CDH1, and SCP3, was remarkably increased.
mRNA expression of Stra8, Odf2, Act, and Prm1 was upregulated in iPS cells by retinoic acid or testosterone induction, whereas Oct-4 transcription was reduced in these cells compared to the controls.
Hormones were also measured in the EB medium.
DNA content analysis by flow cytometry revealed that iPS cells could differentiate into haploid cells through retinoic acid or testosterone treatment.
Collectively, our results suggest that mouse iPS cells possess the potency to differentiate into male germ cells in vitro through embryoid body formation and retinoic acid or testosterone induction.
American Psychological Association (APA)
Li, Peng& Hu, Hongliang& Yang, Shi& Tian, Ruhui& Zhang, Zhenzhen& Zhang, Wei…[et al.]. 2012. Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030712
Modern Language Association (MLA)
Li, Peng…[et al.]. Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030712
American Medical Association (AMA)
Li, Peng& Hu, Hongliang& Yang, Shi& Tian, Ruhui& Zhang, Zhenzhen& Zhang, Wei…[et al.]. Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030712