Bioactive Surface Modification of Hydroxyapatite
Joint Authors
Mizumachi, Wataru
Yasuda, Keisuke
Hirata, Isao
Nogami, Keisuke
Hiasa, Kyou
Okazaki, Yohei
Abe, Yasuhiko
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The purpose of this study was to establish an acid-etching procedure for altering the Ca/P ratio of the nanostructured surface of hydroxyapatite (HAP) by using surface chemical and morphological analyses (XPS, XRD, SEM, surface roughness, and wettability) and to evaluate the in vitro response of osteoblast-like cells (MC3T3-E1 cells) to the modified surfaces.
This study utilized HAP and HAP treated with 10%, 20%, 30%, 40%, 50%, or 60% phosphoric acid solution for 10 minutes at 25°C, followed by rinsing 3 times with ultrapure water.
The 30% phosphoric acid etching process that provided a Ca/P ratio of 1.50, without destruction of the grain boundary of HAP, was selected as a surface-modification procedure.
Additionally, HAP treated by the 30% phosphoric acid etching process was stored under dry conditions at 25°C for 12 hours, and the Ca/P ratio approximated to 1.00 accidentally.
The initial adhesion, proliferation, and differentiation (alkaline phosphatase (ALP) activity and relative mRNA level for ALP) of MC3T3-E1 cells on the modified surfaces were significantly promoted (P<0.05 and 0.01).
These findings show that the 30% phosphoric acid etching process for the nanostructured HAP surface can alter the Ca/P ratio effectively and may accelerate the initial adhesion, proliferation, and differentiation of MC3T3-E1 cells.
American Psychological Association (APA)
Abe, Yasuhiko& Okazaki, Yohei& Hiasa, Kyou& Yasuda, Keisuke& Nogami, Keisuke& Mizumachi, Wataru…[et al.]. 2013. Bioactive Surface Modification of Hydroxyapatite. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030731
Modern Language Association (MLA)
Abe, Yasuhiko…[et al.]. Bioactive Surface Modification of Hydroxyapatite. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030731
American Medical Association (AMA)
Abe, Yasuhiko& Okazaki, Yohei& Hiasa, Kyou& Yasuda, Keisuke& Nogami, Keisuke& Mizumachi, Wataru…[et al.]. Bioactive Surface Modification of Hydroxyapatite. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030731