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Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation
Joint Authors
Ronquillo-Sánchez, M. D.
Fernandez-Mejia, C.
Hernández-Ojeda, S.
Elinos-Baez, M.
Espinosa-Aguirre, Jesús Javier
Camacho-Carranza, Rafael
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Studies have found that biotin favors glucose and lipid metabolism, and medications containing biotin have been developed.
Despite the use of biotin as a pharmacological agent, few studies have addressed toxicity aspects including the possible interaction with cytochrome P450 enzyme family.
This study analyzed the effects of pharmacological doses of biotin on the expression and activity of the cytochrome P4501A subfamily involved in the metabolism of xenobiotics.
Wistar rats were treated daily with biotin (2 mg/kg, i.p.), while the control groups were treated with saline.
All of the rats were sacrificed by cervical dislocation after 1, 3, 5, or 7 days of treatment.
CYP1A1 and CYP1A2 mRNAs were modified by biotin while enzyme activity and protein concentration were not affected.
The lack of an effect of biotin on CYP1A activity was confirmed using other experimental strategies, including (i) cotreatment of the animals with biotin and a known CYP1A inducer; (ii) the addition of biotin to the reaction mixtures for the measurement of CYP1A1 and CYP1A2 activities; and (iii) the use of an S9 mixture that was prepared from control and biotin-treated rats to analyze the activation of benzo[a]pyrene (BaP) into mutagenic metabolites using the Ames test.
The results suggest that biotin does not influence the CYP1A-mediated metabolism of xenobiotics.
American Psychological Association (APA)
Ronquillo-Sánchez, M. D.& Camacho-Carranza, Rafael& Fernandez-Mejia, C.& Hernández-Ojeda, S.& Elinos-Baez, M.& Espinosa-Aguirre, Jesús Javier. 2013. Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030737
Modern Language Association (MLA)
Ronquillo-Sánchez, M. D.…[et al.]. Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030737
American Medical Association (AMA)
Ronquillo-Sánchez, M. D.& Camacho-Carranza, Rafael& Fernandez-Mejia, C.& Hernández-Ojeda, S.& Elinos-Baez, M.& Espinosa-Aguirre, Jesús Javier. Modulation of the Rat Hepatic Cytochrome P4501A Subfamily Using Biotin Supplementation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030737