Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion
Joint Authors
Min, Seung-Ki
Kang, Hyun Ki
Jang, Da Hyun
Jung, Sung Youn
Kim, O. Bok
Yeo, In-Sung
Min, Byung-Il
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated.
The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif).
Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating.
These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR.
On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces.
In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.
American Psychological Association (APA)
Min, Seung-Ki& Kang, Hyun Ki& Jang, Da Hyun& Jung, Sung Youn& Kim, O. Bok& Min, Byung-Il…[et al.]. 2013. Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030753
Modern Language Association (MLA)
Min, Seung-Ki…[et al.]. Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030753
American Medical Association (AMA)
Min, Seung-Ki& Kang, Hyun Ki& Jang, Da Hyun& Jung, Sung Youn& Kim, O. Bok& Min, Byung-Il…[et al.]. Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030753