P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods
Joint Authors
Pasquier, Jennifer
Rioult, Damien
Abu-Kaoud, Nadine
Marie, Sabine
Le Foll, Frank
Guerrouahen, Bella S.
Tabrizi, Arash Rafii
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
P-gp expression has been linked to the efflux of chemotherapeutic drugs in human cancers leading to multidrug resistance.
Fluorescence techniques have been widely applied to measure the P-gp activity.
In this paper, there is a comparison between the advantages of two fluorescence approaches of commonly available and affordable instruments: the microplate reader (MPR) and the flow cytometer to detect the P-gp efflux activity using calcein-AM.
Results.
The selectivity, sensibility, and reproducibility of the two methods have been defined.
Our results showed that the MPR is more powerful for the detection of small inhibition, whereas the flow cytometry method is more reliable at higher concentrations of the inhibitors.
We showed that to determine precisely the inhibition efficacy the flow cytometry is better; hence, to get the correct Emax and EC50 values, we cannot only rely on the MPR.
Conclusion.
Both techniques can potentially be used extensively in the pharmaceutical industry for high-throughput drug screening and in biology laboratories for academic research, monitoring the P-gp efflux in specific assays.
American Psychological Association (APA)
Pasquier, Jennifer& Rioult, Damien& Abu-Kaoud, Nadine& Marie, Sabine& Tabrizi, Arash Rafii& Guerrouahen, Bella S.…[et al.]. 2013. P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1030776
Modern Language Association (MLA)
Pasquier, Jennifer…[et al.]. P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1030776
American Medical Association (AMA)
Pasquier, Jennifer& Rioult, Damien& Abu-Kaoud, Nadine& Marie, Sabine& Tabrizi, Arash Rafii& Guerrouahen, Bella S.…[et al.]. P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1030776
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030776