Recombinant Rat CC10 Protein Inhibits PDGF-Induced Airway Smooth Muscle Cells Proliferation and Migration
Joint Authors
Xu, Yu-Dong
Wei, Ying
Liu, Qi
Ma, Zi-Feng
Yang, Yong Qing
Lui, Yan-Yan
Yin, Lei-Miao
Ran, Jun
Wang, Yu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Abnormal migration and proliferation of airway smooth muscle cells (ASMCs) in the airway cause airway wall thickening, which is strongly related with the development of airway remodeling in asthma.
Clara cell 10 kDa protein (CC10), which is secreted by the epithelial clara cells of the pulmonary airways, plays an important role in the regulation of immunological and inflammatory processes.
Previous studies suggested that CC10 protein had great protective effects against inflammation in asthma.
However, the effects of CC10 protein on ASMCs migration and proliferation in airway remodeling were poorly understood.
In this study, we constructed the pET-22b-CC10 recombinant plasmid, induced expression and purified the recombinant rat CC10 protein from E.
coli by Ni2+ affinity chromatography and ion exchange chromatography purification.
We investigated the effect of recombinant rat CC10 protein on platelet-derived growth factor (PDGF)-BB-induced ASMCs proliferation and migration.
Our results demonstrated that the recombinant CC10 protein could inhibit PDGF-BB-induced cell viability, proliferation and migration.
Western blot analysis showed that PDGF-BB-induced activation of cyclin D1 was inhibited by CC10.
These findings implicated that CC10 could inhibit increased ASMCs proliferation, and migration induced by PDGF-BB, and this suppression effect might be associated with inhibition of cyclin D1 expression, which might offer hope for the future treatment of airway remodeling.
American Psychological Association (APA)
Wei, Ying& Xu, Yu-Dong& Yin, Lei-Miao& Wang, Yu& Ran, Jun& Liu, Qi…[et al.]. 2013. Recombinant Rat CC10 Protein Inhibits PDGF-Induced Airway Smooth Muscle Cells Proliferation and Migration. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030798
Modern Language Association (MLA)
Wei, Ying…[et al.]. Recombinant Rat CC10 Protein Inhibits PDGF-Induced Airway Smooth Muscle Cells Proliferation and Migration. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030798
American Medical Association (AMA)
Wei, Ying& Xu, Yu-Dong& Yin, Lei-Miao& Wang, Yu& Ran, Jun& Liu, Qi…[et al.]. Recombinant Rat CC10 Protein Inhibits PDGF-Induced Airway Smooth Muscle Cells Proliferation and Migration. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030798