Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs
Joint Authors
Astegno, Alessandra
Allegrini, Alessandra
Dominici, Paola
Cellini, Barbara
Giorgetti, Alejandro
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The emergence of antibiotic resistance in microbial pathogens requires the identification of new antibacterial drugs.
The biosynthesis of methionine is an attractive target because of its central importance in cellular metabolism.
Moreover, most of the steps in methionine biosynthesis pathway are absent in mammals, lowering the probability of unwanted side effects.
Herein, detailed biochemical characterization of one enzyme required for methionine biosynthesis, a pyridoxal-5′-phosphate (PLP-) dependent C-S lyase from Corynebacterium diphtheriae, a pathogenic bacterium that causes diphtheria, has been performed.
We overexpressed the protein in E.
coli and analyzed substrate specificity, pH dependence of steady state kinetic parameters, and ligand-induced spectral transitions of the protein.
Structural comparison of the enzyme with cystalysin from Treponema denticola indicates a similarity in overall folding.
We used site-directed mutagenesis to highlight the importance of active site residues Tyr55, Tyr114, and Arg351, analyzing the effects of amino acid replacement on catalytic properties of enzyme.
Better understanding of the active site of C.
diphtheriae C-S lyase and the determinants of substrate and reaction specificity from this work will facilitate the design of novel inhibitors as antibacterial therapeutics.
American Psychological Association (APA)
Astegno, Alessandra& Giorgetti, Alejandro& Allegrini, Alessandra& Cellini, Barbara& Dominici, Paola. 2013. Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030810
Modern Language Association (MLA)
Astegno, Alessandra…[et al.]. Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1030810
American Medical Association (AMA)
Astegno, Alessandra& Giorgetti, Alejandro& Allegrini, Alessandra& Cellini, Barbara& Dominici, Paola. Characterization of C-S Lyase from C. diphtheriae: A Possible Target for New Antimicrobial Drugs. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030810