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Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia
Joint Authors
Sapienza, Maria Rosaria
Gazzola, Anna
Righi, Simona
Piccaluga, Pier Paolo
Fuligni, Fabio
Ciccone, Maria
Luciani, Marco
Rigolin, Gian Matteo
Rossi, Maura
Mannu, Claudia
Laginestra, Maria Antonella
Cuneo, Antonio
Agostinelli, Claudio
Pileri, Stefano A.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) is the commonest leukemia in adults.
Here, we aimed to evaluate hsa-miR-15a/hsa-miR-16-1 expression in CLL tissues by qPCR and correlate it with the other clinicopathological features and clinical outcome.
40 formalin-fixed paraffin-embedded (FFPE) lymph node samples obtained from CLL/SLL patients were classified into two categories, “PCs rich” and “typical.” We found a significant common expression level of 4 miRNAs; however, we did not find any significant relationship between PCs presence and miRNAs expression.
Moreover, neither the presence of 13q deletion nor the percentage of cells carrying the deletion strictly correlated with miRNAs expression levels, although a significant number of patients with 13q deletion presented hsa-miR-16-1-3p levels below the median value in normal samples (P<0.05).
Finally, although no correlation was found between the expression of each miRNA and other clinicopathological features (Ki67, CD38, ZAP70, and IGVH@ hypermutations), the OS curves showed a positive trend in patients with miRNAs downregulation, though not statistically significant.
In conclusion, we showed for the first time that all miRNAs can be successfully studied in FFPE CLL tissues and that del13q and PCs richness do not strictly correspond to miRNAs downregulation; therefore, a specific evaluation may be envisaged at least in patients enrolled in clinical trials.
American Psychological Association (APA)
Rossi, Maura& Fuligni, Fabio& Ciccone, Maria& Agostinelli, Claudio& Righi, Simona& Luciani, Marco…[et al.]. 2013. Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030829
Modern Language Association (MLA)
Rossi, Maura…[et al.]. Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1030829
American Medical Association (AMA)
Rossi, Maura& Fuligni, Fabio& Ciccone, Maria& Agostinelli, Claudio& Righi, Simona& Luciani, Marco…[et al.]. Hsa-miR-15a and Hsa-miR-16-1 Expression Is Not Related to Proliferation Centers Abundance and Other Prognostic Factors in Chronic Lymphocytic Leukemia. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1030829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030829