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Altered mRNA Expression Related to the Apoptotic Effect of Three Xanthones on Human Melanoma SK-MEL-28 Cell Line
Joint Authors
Zhang, Wei
Wang, Jing J.
Sanderson, Barbara J. S.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We previously demonstrated that α-mangostin, γ-mangostin, and 8-deoxygartanin have significant cytotoxic effects on human melanoma SK-MEL-28 cell line.
The current study revealed the underlying mechanisms.
α-Mangostin (7.5 μg/mL) activated caspase activity, with a 3-fold and 4-fold increased caspase 8 and 9 activity, respectively.
The molecular mechanisms were investigated by qRT-PCR for mRNA related to cell cycle arrest in G1 phase (p21WAF1 and cyclin D1), apoptosis (cytochrome C, Bcl-2, and Bax), and survival pathways (Akt1, NFκB, and IκBα).
α-Mangostin significantly upregulated mRNA expression of cytochrome C and p21WAF1 and downregulated that of cyclin D1, Akt1, and NFκB.
γ-Mangostin significantly downregulated mRNA expression of Akt1 and NFκB and upregulated p21WAF1 and IκBα.
8-Deoxygartanin significantly upregulated the mRNA expression of p21WAF1 and downregulated that of cyclin D1 and NFκB.
The three xanthones significantly inhibited the mRNA expression of the BRAF V600E mutation.
Moreover, α-mangostin and γ-mangostin significantly downregulated Akt phosphorylation at Ser473.
In conclusion, the three xanthones induced an inhibitory effect on SK-MEL-28 cells by modulating the molecular targets involved in the apoptotic pathways.
American Psychological Association (APA)
Wang, Jing J.& Zhang, Wei& Sanderson, Barbara J. S.. 2013. Altered mRNA Expression Related to the Apoptotic Effect of Three Xanthones on Human Melanoma SK-MEL-28 Cell Line. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030831
Modern Language Association (MLA)
Wang, Jing J.…[et al.]. Altered mRNA Expression Related to the Apoptotic Effect of Three Xanthones on Human Melanoma SK-MEL-28 Cell Line. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030831
American Medical Association (AMA)
Wang, Jing J.& Zhang, Wei& Sanderson, Barbara J. S.. Altered mRNA Expression Related to the Apoptotic Effect of Three Xanthones on Human Melanoma SK-MEL-28 Cell Line. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030831