Suppression of Osteopontin Functions by Levocetirizine, a Histamine H1 Receptor Antagonist, In Vitro
Joint Authors
Suzaki, Harumi
Asano, Kazuhito
Komatsuzaki, Toshimitsu
Suzaki, Isao
Hirano, Kojiro
Kanai, Ken-Ichi
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objectives.
Osteopontin (OPN), a multifunctional glycoprotein secreted from a wide variety of cells after inflammatory stimulation, is well accepted to contribute to the development of allergic diseases.
However, the influence of histamine H1 receptor antagonists (antihistamines) on OPN functions is not well understood.
The present study was undertaken to examine the influence of antihistamines on OPN functions in vitro.
Methods.
Human nasal epithelial cells (5×105 cells) were stimulated with 250 ng/mL OPN in the presence of either desloratadine (DL), fexofenadine (FEX), or levocetirizine (LCT).
The levels of OPN, GM-CSF, Eotaxin, and RANTES in 24 h culture supernatants were examined by ELISA.
The influence of LCT on mRNA expression and transcription factor activation in cells were also examined by real-time RT-PCR and ELISA, respectively.
Key Findings.
The antihistamines examined significantly suppressed the production of GM-CSF, Eotaxin, and RANTES from cells after OPN stimulation.
LCT also exhibited the suppression of mRNA expression for chemokines and transcription factor, NF-κB and AP-1, activation, which were increased by the stimulation of cells with OPN.
Conclusions.
The suppressive activity of LCT on OPN functions on nasal epithelial cells may be responsible for the attenuating effect of the agent on allergic diseases.
American Psychological Association (APA)
Komatsuzaki, Toshimitsu& Suzaki, Isao& Hirano, Kojiro& Kanai, Ken-Ichi& Asano, Kazuhito& Suzaki, Harumi. 2013. Suppression of Osteopontin Functions by Levocetirizine, a Histamine H1 Receptor Antagonist, In Vitro. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030858
Modern Language Association (MLA)
Komatsuzaki, Toshimitsu…[et al.]. Suppression of Osteopontin Functions by Levocetirizine, a Histamine H1 Receptor Antagonist, In Vitro. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030858
American Medical Association (AMA)
Komatsuzaki, Toshimitsu& Suzaki, Isao& Hirano, Kojiro& Kanai, Ken-Ichi& Asano, Kazuhito& Suzaki, Harumi. Suppression of Osteopontin Functions by Levocetirizine, a Histamine H1 Receptor Antagonist, In Vitro. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030858