![](/images/graphics-bg.png)
TrkAIII Promotes Microtubule Nucleation and Assembly at the Centrosome in SH-SY5Y Neuroblastoma Cells, Contributing to an Undifferentiated Anaplastic Phenotype
Joint Authors
Gulino, Alberto
Farina, Antonietta R.
Di Ianni, Natalia
Cappabianca, Lucia
Ruggeri, Pierdomenico
Ragone, Marzia
Ianni, Giulia
Mackay, Andrew R.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The alternative TrkAIII splice variant is expressed by advanced stage human neuroblastomas (NBs) and exhibits oncogenic activity in NB models.
In the present study, employing stable transfected cell lines and assays of indirect immunofluorescence, immunoprecipitation, Western blotting, microtubule regrowth, tubulin kinase, and tubulin polymerisation, we report that TrkAIII binds α-tubulin and promotes MT nucleation and assembly at the centrosome.
This effect depends upon spontaneous TrkAIII activity, TrkAIII localisation to the centrosome and pericentrosomal area, and the capacity of TrkAIII to bind, phosphorylate, and polymerise tubulin.
We propose that this novel role for TrkAIII contributes to MT involvement in the promotion and maintenance of an undifferentiated anaplastic NB cell morphology by restricting and augmenting MT nucleation and assembly at the centrosomal MTOC.
American Psychological Association (APA)
Farina, Antonietta R.& Di Ianni, Natalia& Cappabianca, Lucia& Ruggeri, Pierdomenico& Ragone, Marzia& Ianni, Giulia…[et al.]. 2013. TrkAIII Promotes Microtubule Nucleation and Assembly at the Centrosome in SH-SY5Y Neuroblastoma Cells, Contributing to an Undifferentiated Anaplastic Phenotype. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030861
Modern Language Association (MLA)
Farina, Antonietta R.…[et al.]. TrkAIII Promotes Microtubule Nucleation and Assembly at the Centrosome in SH-SY5Y Neuroblastoma Cells, Contributing to an Undifferentiated Anaplastic Phenotype. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030861
American Medical Association (AMA)
Farina, Antonietta R.& Di Ianni, Natalia& Cappabianca, Lucia& Ruggeri, Pierdomenico& Ragone, Marzia& Ianni, Giulia…[et al.]. TrkAIII Promotes Microtubule Nucleation and Assembly at the Centrosome in SH-SY5Y Neuroblastoma Cells, Contributing to an Undifferentiated Anaplastic Phenotype. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030861