The Short ITS2 Sequence Serves as an Efficient Taxonomic Sequence Tag in Comparison with the Full-Length ITS
Joint Authors
Chen, Shi-Lin
Han, Jianping
Zhu, Yingjie
Chen, Xiaochen
Liao, Baoshen
Yao, Hui
Meng, Fanyun
Song, Jingyuan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An ideal DNA barcoding region should be short enough to be amplified from degraded DNA.
In this paper, we discuss the possibility of using a short nuclear DNA sequence as a barcode to identify a wide range of medicinal plant species.
First, the PCR and sequencing success rates of ITS and ITS2 were evaluated based entirely on materials from dry medicinal product and herbarium voucher specimens, including some samples collected back to 90 years ago.
The results showed that ITS2 could recover 91% while ITS could recover only 23% efficiency of PCR and sequencing by using one pair of primer.
Second, 12861 ITS and ITS2 plant sequences were used to compare the identification efficiency of the two regions.
Four identification criteria (BLAST, inter- and intradivergence Wilcoxon signed rank tests, and TaxonDNA) were evaluated.
Our results supported the hypothesis that ITS2 can be used as a minibarcode to effectively identify species in a wide variety of specimens and medicinal materials.
American Psychological Association (APA)
Han, Jianping& Zhu, Yingjie& Chen, Xiaochen& Liao, Baoshen& Yao, Hui& Song, Jingyuan…[et al.]. 2013. The Short ITS2 Sequence Serves as an Efficient Taxonomic Sequence Tag in Comparison with the Full-Length ITS. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030865
Modern Language Association (MLA)
Han, Jianping…[et al.]. The Short ITS2 Sequence Serves as an Efficient Taxonomic Sequence Tag in Comparison with the Full-Length ITS. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030865
American Medical Association (AMA)
Han, Jianping& Zhu, Yingjie& Chen, Xiaochen& Liao, Baoshen& Yao, Hui& Song, Jingyuan…[et al.]. The Short ITS2 Sequence Serves as an Efficient Taxonomic Sequence Tag in Comparison with the Full-Length ITS. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030865