Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier
Joint Authors
Amini, Razieh
Azizi Jalilian, Farid
Veerakumarasivam, Abhi
Abdulamir, Ahmed S.
Nadali, Fatemeh
Rosli, Rozita
Abdullah, Syahril
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML).
Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF.
This study investigates the outcome of delivery of a VEGF165 antagonist, soluble vascular endothelial growth factor receptor, namely sFLT-1, mediating lipofectamine 2000 in acute myeloid leukemic cells.
A recombinant plasmid expressing sFLT-1 was constructed and transfected into the K562 and HL60 cells using lipofectamine 2000 transfection reagent.
sFLT-1 expression/secretion in pVAX-sFLT-1 transfected cells was verified by RT-PCR and western blot.
MTS assay was carried out to evaluate the effect of sFLT-1 on human umbilical vein endothelial cells and K562 and HL60 cells in vitro.
Treatment with pVAX-sFLT-1 showed no association between sFLT-1 and proliferation of infected K562 and HL60 cells, while it demonstrated a significant inhibitory impact on the proliferation of HUVECs.
The results of the current study imply that the combination of nonviral gene carrier and sFLT-1 possesses the potential to provide efficient tool for the antiangiogenic gene therapy of AML.
American Psychological Association (APA)
Amini, Razieh& Azizi Jalilian, Farid& Veerakumarasivam, Abhi& Abdullah, Syahril& Abdulamir, Ahmed S.& Nadali, Fatemeh…[et al.]. 2013. Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030876
Modern Language Association (MLA)
Amini, Razieh…[et al.]. Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030876
American Medical Association (AMA)
Amini, Razieh& Azizi Jalilian, Farid& Veerakumarasivam, Abhi& Abdullah, Syahril& Abdulamir, Ahmed S.& Nadali, Fatemeh…[et al.]. Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030876
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030876