An Experimental Approach for Selecting Appropriate Rodent Diets for Research Studies on Metabolic Disorders
Joint Authors
Agarwal, Amit
Sasidharan, Suja Rani
Anandakumar, Senthilkumar
Venkatesan, Vijayabalaji
Ariyattu Madhavan, Chandrasekharan Nair
Allan, Joseph Joshua
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Diverse high energy diets have been utilized to precipitate obesity and related metabolic disorders in rodent models, though the dietary intervention has not absolutely been standardized.
The present study established usage of a customized semipurified normal control diet (NCD) and high fat diet (HFD), for research studies on diet-induced metabolic disorders in albino rats.
Male Wistar rats were fed with normal pellet diet (NPD) or customized NCDs I, II, III or HFDs I, II, III for 12 weeks and parameters, namely, body weight, visceral adiposity, serum triglycerides, cholesterol, and glucose were evaluated to select an appropriate NCD and HFD.
The selected HFD was further evaluated for induction of fatty liver, whilst type 2 diabetes (T2D) induction was confirmed in HFD and streptozotocin (STZ) induced diabetes model in Wistar rats.
Amongst different diets tested, NCD-I and HFD-I were selected, since NCD-I exhibited close resemblance to NPD, whereas HFD-I induced metabolic alterations, particularly obesity and dyslipidemia consistently.
Moreover, HFD-I elevated terminal hepatic lipids, while HFD-I/STZ treatment augmented insulin resistance index and serum glucose levels significantly indicating effective induction of fatty liver and T2D, respectively.
Therefore, customized semipurified NCD-I and HFD-I can be recommended for research studies on diet-induced metabolic disorders in albino Wistar rats.
American Psychological Association (APA)
Sasidharan, Suja Rani& Allan, Joseph Joshua& Anandakumar, Senthilkumar& Venkatesan, Vijayabalaji& Ariyattu Madhavan, Chandrasekharan Nair& Agarwal, Amit. 2013. An Experimental Approach for Selecting Appropriate Rodent Diets for Research Studies on Metabolic Disorders. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030877
Modern Language Association (MLA)
Sasidharan, Suja Rani…[et al.]. An Experimental Approach for Selecting Appropriate Rodent Diets for Research Studies on Metabolic Disorders. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030877
American Medical Association (AMA)
Sasidharan, Suja Rani& Allan, Joseph Joshua& Anandakumar, Senthilkumar& Venkatesan, Vijayabalaji& Ariyattu Madhavan, Chandrasekharan Nair& Agarwal, Amit. An Experimental Approach for Selecting Appropriate Rodent Diets for Research Studies on Metabolic Disorders. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030877
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030877