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Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats
Joint Authors
Simeonova, Rumyana
Krasteva, Ilina
Manov, Vassil
Mitcheva, Mitka
Vitcheva, Vessela
Kondeva-Burdina, Magdalena
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The hepatoprotective potential of saponarin, isolated from Gypsophila trichotoma, was evaluated in vitro/in vivo using a hepatotoxicity model of paracetamol-induced liver injury.
In freshly isolated rat hepatocytes, paracetamol (100 μmol) led to a significant decrease in cell viability, increased LDH leakage, decreased levels of cellular GSH, and elevated MDA quantity.
Saponarin (60–0.006 μg/mL) preincubation, however, significantly ameliorated paracetamol-induced hepatotoxicity in a concentration-dependent manner.
The beneficial effect of saponarin was also observed in vivo.
Rats were challenged with paracetamol alone (600 mg/kg, i.p.) and after 7-day pretreatment with saponarin (80 mg/kg, oral gavage).
Paracetamol toxicity was evidenced by increase in MDA quantity and decrease in cell GSH levels and antioxidant defence system.
No changes in phase I enzyme activities of AH and EMND and cytochrome P 450 quantity were detected.
Saponarin pretreatment resulted in significant increase in cell antioxidant defence system and GSH levels and decrease in lipid peroxidation.
The biochemical changes are in good correlation with the histopathological data.
Protective activity of saponarin was similar to the activity of positive control silymarin.
On the basis of these results, it can be concluded that saponarin exerts antioxidant and hepatoprotective activity against paracetamol liver injury in vitro/in vivo.
American Psychological Association (APA)
Simeonova, Rumyana& Vitcheva, Vessela& Kondeva-Burdina, Magdalena& Krasteva, Ilina& Manov, Vassil& Mitcheva, Mitka. 2013. Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030881
Modern Language Association (MLA)
Simeonova, Rumyana…[et al.]. Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1030881
American Medical Association (AMA)
Simeonova, Rumyana& Vitcheva, Vessela& Kondeva-Burdina, Magdalena& Krasteva, Ilina& Manov, Vassil& Mitcheva, Mitka. Hepatoprotective and Antioxidant Effects of Saponarin, Isolated from Gypsophila trichotoma Wend. on Paracetamol-Induced Liver Damage in Rats. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1030881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030881