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Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods
Joint Authors
Tuononen, Katja
Sarhadi, Virinder Kaur
Wirtanen, Aino
Rönty, Mikko
Salmenkivi, Kaisa
Knuuttila, Aija
Remes, Satu
Telaranta-Keerie, Aino I.
Bloor, Stuart
Ellonen, Pekka
Knuutila, Sakari
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Anaplastic lymphoma receptor tyrosine kinase (ALK) gene rearrangements occur in a subgroup of non-small cell lung carcinomas (NSCLCs).
The identification of these rearrangements is important for guiding treatment decisions.
The aim of our study was to screen ALK gene fusions in NSCLCs and to compare the results detected by targeted resequencing with results detected by commonly used methods, including fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and real-time reverse transcription-PCR (RT-PCR).
Furthermore, we aimed to ascertain the potential of targeted resequencing in detection of ALK-rearranged lung carcinomas.
We assessed ALK fusion status for 95 formalin-fixed paraffin-embedded tumor tissue specimens from 87 patients with NSCLC by FISH and real-time RT-PCR, for 57 specimens from 56 patients by targeted resequencing, and for 14 specimens from 14 patients by IHC.
All methods were performed successfully on formalin-fixed paraffin-embedded tumor tissue material.
We detected ALK fusion in 5.7% (5 out of 87) of patients examined.
The results obtained from resequencing correlated significantly with those from FISH, real-time RT-PCR, and IHC.
Targeted resequencing proved to be a promising method for ALK gene fusion detection in NSCLC.
Means to reduce the material and turnaround time required for analysis are, however, needed.
American Psychological Association (APA)
Tuononen, Katja& Sarhadi, Virinder Kaur& Wirtanen, Aino& Rönty, Mikko& Salmenkivi, Kaisa& Knuuttila, Aija…[et al.]. 2013. Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030883
Modern Language Association (MLA)
Tuononen, Katja…[et al.]. Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030883
American Medical Association (AMA)
Tuononen, Katja& Sarhadi, Virinder Kaur& Wirtanen, Aino& Rönty, Mikko& Salmenkivi, Kaisa& Knuuttila, Aija…[et al.]. Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030883