Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran
Joint Authors
Mohebali, Mehdi
Hajjaran, Homa
Mamishi, Setareh
Vasigheh, Farzaneh
Oshaghi, Mohammad Ali
Naddaf, Saied Reza
Teimouri, Aref
Edrissian, Gholam Hossein
Zarei, Zabiholah
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Amplification of internal transcript spacer 1 of ribosomal RNA (ITS1-RNA) gene followed by RFLP analysis and sequencing was used to identify the causing agents of cutaneous and visceral leishmaniasis (CL and VL) in humans and animal reservoir hosts from various geographical areas in Iran.
We also used random amplified polymorphic DNA (RAPD-PCR) to obtain polymorphisms among isolates of Leishmania spp.
Totally, 362 suspected human and animal cases including 173 CL, 49 VL, 60 rodents, and 80 domestic dogs were examined for Leishmania infection.
From 112 culture-positive samples prepared from CL cases, 75 (67%) were infected with L.
major and 37 (33%) with L.
tropica.
Of the 60 rodents examined, 25 (41.6%) harbored the Leishmania infection; 21 were infected with L.
major and 4 with L.
turanica.
From 49 suspected VL, 29 were positive by direct agglutination test (DAT), whereas microscopy detected parasite in bone marrow of 25 and culture in 28 of the patients.
Two VL patients were infected with L.
tropica and 26 with L.
infantum.
Of the 80 domestic dogs, 56 showed anti-Leishmania antibodies with DAT.
Of these, 55 were positive by both microscopy and culture.
Molecular identity, obtained only for 47 samples, revealed L.
infantum in 43 and L.
tropica in 4 dogs.
The polymorphisms among L.
tropica and L.
major isolates were 3.6% and 7.3%; the rate among human and canine VL isolates was 2.8% and 9.8%, respectively.
Our results showed that at least four different Leishmania species with various polymorphisms circulate among humans and animal hosts in Iran.
American Psychological Association (APA)
Hajjaran, Homa& Mohebali, Mehdi& Mamishi, Setareh& Vasigheh, Farzaneh& Oshaghi, Mohammad Ali& Naddaf, Saied Reza…[et al.]. 2013. Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030914
Modern Language Association (MLA)
Hajjaran, Homa…[et al.]. Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1030914
American Medical Association (AMA)
Hajjaran, Homa& Mohebali, Mehdi& Mamishi, Setareh& Vasigheh, Farzaneh& Oshaghi, Mohammad Ali& Naddaf, Saied Reza…[et al.]. Molecular Identification and Polymorphism Determination of Cutaneous and Visceral Leishmaniasis Agents Isolated from Human and Animal Hosts in Iran. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1030914
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030914