Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury
Joint Authors
Ibarra, Antonio
García, Elisa
Flores-Romero, Adrian
Mestre, Humberto
Flores, Carmina
Rodríguez-Barrera, Roxana
Fernández-Presas, Ana M.
Martiñón, Susana
González-Puertos, Viridiana Yazmín
Rodriguez-Mata, Verónica
Solano, Sandra
Konigsberg, Mina
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Apoptosis is one of the most destructive mechanisms that develop after spinal cord (SC) injury.
Immunization with neural-derived peptides (INDPs) such as A91 has shown to reduce the deleterious proinflammatory response and the amount of harmful compounds produced after SC injury.
With the notion that the aforementioned elements are apoptotic inducers, we hypothesized that INDPs would reduce apoptosis after SC injury.
In order to test this assumption, adult rats were subjected to SC contusion and immunized either with A91 or phosphate buffered saline (PBS; control group).
Seven days after injury, animals were euthanized to evaluate the number of apoptotic cells at the injury site.
Apoptosis was evaluated using DAPI and TUNEL techniques; caspase-3 activity was also evaluated.
To further elucidate the mechanisms through which A91 exerts this antiapoptotic effects we quantified tumor necrosis factor-alpha (TNF-α).
To also demonstrate that the decrease in apoptotic cells correlated with a functional improvement, locomotor recovery was evaluated.
Immunization with A91 significantly reduced the number of apoptotic cells and decreased caspase-3 activity and TNF-α concentration.
Immunization with A91 also improved the functional recovery of injured rats.
The present study shows the beneficial effect of INDPs on preventing apoptosis and provides more evidence on the neuroprotective mechanisms exerted by this strategy.
American Psychological Association (APA)
Rodríguez-Barrera, Roxana& Fernández-Presas, Ana M.& García, Elisa& Flores-Romero, Adrian& Martiñón, Susana& González-Puertos, Viridiana Yazmín…[et al.]. 2013. Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030961
Modern Language Association (MLA)
Rodríguez-Barrera, Roxana…[et al.]. Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030961
American Medical Association (AMA)
Rodríguez-Barrera, Roxana& Fernández-Presas, Ana M.& García, Elisa& Flores-Romero, Adrian& Martiñón, Susana& González-Puertos, Viridiana Yazmín…[et al.]. Immunization with a Neural-Derived Peptide Protects the Spinal Cord from Apoptosis after Traumatic Injury. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030961
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030961