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A Comprehensive Tutorial on In Vitro Characterization of New Photosensitizers for Photodynamic Antitumor Therapy and Photodynamic Inactivation of Microorganisms
Joint Authors
Berneburg, Mark
Plaetzer, Kristjan
Maisch, Tim
Gollmer, Anita
Kiesslich, Tobias
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-16
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
In vitro research performed on eukaryotic or prokaryotic cell cultures usually represents the initial step for characterization of a novel photosensitizer (PS) intended for application in photodynamic therapy (PDT) of cancer or photodynamic inactivation (PDI) of microorganisms.
Although many experimental steps of PS testing make use of the wide spectrum of methods readily employed in cell biology, special aspects of working with photoactive substances, such as the autofluorescence of the PS molecule or the requirement of light protection, need to be considered when performing in vitro experiments in PDT/PDI.
This tutorial represents a comprehensive collection of operative instructions, by which, based on photochemical and photophysical properties of a PS, its uptake into cells, the intracellular localization and photodynamic action in both tumor cells and microorganisms novel photoactive molecules may be characterized for their suitability for PDT/PDI.
Furthermore, it shall stimulate the efforts to expand the convincing benefits of photodynamic therapy and photodynamic inactivation within both established and new fields of applications and motivate scientists of all disciplines to get involved in photodynamic research.
American Psychological Association (APA)
Kiesslich, Tobias& Gollmer, Anita& Maisch, Tim& Berneburg, Mark& Plaetzer, Kristjan. 2013. A Comprehensive Tutorial on In Vitro Characterization of New Photosensitizers for Photodynamic Antitumor Therapy and Photodynamic Inactivation of Microorganisms. BioMed Research International،Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-1030988
Modern Language Association (MLA)
Kiesslich, Tobias…[et al.]. A Comprehensive Tutorial on In Vitro Characterization of New Photosensitizers for Photodynamic Antitumor Therapy and Photodynamic Inactivation of Microorganisms. BioMed Research International No. 2013 (2013), pp.1-17.
https://search.emarefa.net/detail/BIM-1030988
American Medical Association (AMA)
Kiesslich, Tobias& Gollmer, Anita& Maisch, Tim& Berneburg, Mark& Plaetzer, Kristjan. A Comprehensive Tutorial on In Vitro Characterization of New Photosensitizers for Photodynamic Antitumor Therapy and Photodynamic Inactivation of Microorganisms. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-1030988
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1030988