7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1
Joint Authors
Kim, Ki Cheon
Lee, In Kyung
Cha, Ji Won
Cho, Suk Ju
Na, Soo Young
Chae, Sungwook
Kim, Hye Sun
Kim, Suhkmann
Hyun, Jin Won
Kang, Kyoung Ah
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The modified guanine base 8-oxoguanine (8-oxoG) is abundantly produced by oxidative stress, can contribute to carcinogenesis, and can be removed from DNA by 8-oxoguanine DNA glycosylase-1 (OGG1), which acts as an 8-oxoG glycosylase and endonuclease.
This study investigated the mechanism by which 7,8-dihydroxyflavone (DHF) inhibits oxidative stress-induced 8-oxoG formation in hamster lung fibroblasts (V79-4).
DHF significantly reduced the amount of 8-oxoG induced by hydrogen peroxide (H2O2) and elevated the levels of OGG1 mRNA and protein.
DHF increased the binding of nuclear factor erythroid 2-related factor 2 (Nrf2) to antioxidant response element sequences in the upstream promoter region of OGG1.
Moreover, DHF increased the nuclear levels of Nrf2, small Maf proteins, and the Nrf2/small Maf complex, all of which are decreased by H2O2 treatment.
Likewise, the level of phosphorylated Akt, which activates Nrf2, was decreased by H2O2 treatment but restored by DHF treatment.
The levels of OGG1 and nuclear translocation of Nrf2 protein were decreased upon treatment with PI3K inhibitor or Akt inhibitor, and DHF treatment did not restore OGG1 and nuclear Nrf2 levels in these inhibitor-treated cells.
Furthermore, PI3K and Akt inhibitors abolished the protective effects of DHF in cells undergoing oxidative stress.
These data indicate that DHF induces OGG1 expression via the PI3K-Akt pathway and protects cells against oxidative DNA base damage by activating DNA repair systems.
American Psychological Association (APA)
Kim, Ki Cheon& Lee, In Kyung& Kang, Kyoung Ah& Cha, Ji Won& Cho, Suk Ju& Na, Soo Young…[et al.]. 2013. 7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1031035
Modern Language Association (MLA)
Kim, Ki Cheon…[et al.]. 7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1031035
American Medical Association (AMA)
Kim, Ki Cheon& Lee, In Kyung& Kang, Kyoung Ah& Cha, Ji Won& Cho, Suk Ju& Na, Soo Young…[et al.]. 7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1031035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031035