Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients
Joint Authors
Kan, Wei-Chih
Chien, Chih-Chiang
Liao, Alex Chien Hwa
Liao, Pao-Chi
Wang, Hsien-Yi
Wu, Hsin-Yi
Huang, Chien-Cheng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Peritoneal dialysis (PD) frequently contributes to peritoneal damage which cannot be easily identified without invasive techniques, implying the urgent need for biomarkers and revealing mechanisms.
Chronic glomerulonephritis (CGN) is one of the leading causes of receiving dialysis treatment.
Here, we attempted to analyze the peritoneal dialysate collected from CGN patients when they receive continuous ambulatory peritoneal dialysis (CAPD) treatment for the first time and after a year to reveal the protein changes that resulted from PD.
Proteins were displayed by two-dimensional gel electrophoresis (2DE).
Altered gel spots were digested followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification.
Eight proteins were found to have differential expression levels between two groups.
Their differential expressions were validated by Western blots in other sets of peritoneal dialysates.
Proteins identified with higher levels in the first-time dialysate suggested their dominant appearance in CGN patients, while those that showed higher levels in peritoneal dialysate collected after one year may result from initial peritoneal inflammation or changes in the permeability of the peritoneum to middle-sized proteins.
All the identified proteins may provide a perceptiveness of peritoneal changes caused by PD and may function as potential biomarkers or drug targets.
American Psychological Association (APA)
Wu, Hsin-Yi& Liao, Alex Chien Hwa& Huang, Chien-Cheng& Liao, Pao-Chi& Chien, Chih-Chiang& Kan, Wei-Chih…[et al.]. 2013. Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1031036
Modern Language Association (MLA)
Wu, Hsin-Yi…[et al.]. Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1031036
American Medical Association (AMA)
Wu, Hsin-Yi& Liao, Alex Chien Hwa& Huang, Chien-Cheng& Liao, Pao-Chi& Chien, Chih-Chiang& Kan, Wei-Chih…[et al.]. Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1031036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031036