Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells
Joint Authors
Muñoz, Maria de Lourdes
Limón-Camacho, Gustavo
Tovar, Rosalinda
Diaz-Badillo, Alvaro
Black, William C.
Mendoza-Hernández, Guillermo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells.
The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors.
In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae.
aegypti.
To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed.
Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa.
In addition, viral particles bound high molecular weight proteins.
Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin.
American Psychological Association (APA)
Muñoz, Maria de Lourdes& Limón-Camacho, Gustavo& Tovar, Rosalinda& Diaz-Badillo, Alvaro& Mendoza-Hernández, Guillermo& Black, William C.. 2013. Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031050
Modern Language Association (MLA)
Muñoz, Maria de Lourdes…[et al.]. Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1031050
American Medical Association (AMA)
Muñoz, Maria de Lourdes& Limón-Camacho, Gustavo& Tovar, Rosalinda& Diaz-Badillo, Alvaro& Mendoza-Hernández, Guillermo& Black, William C.. Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031050