In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene
Joint Authors
Sato, Kenzo
Saito, Rumiko
Taniguchi, Makoto
Oda, Haruka
Soma, Atsumi
Yamane, Mariko
Nakanishi, Tomoko
Yasunaga, Mayu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Vitamin D is an essential factor for ossification, and its deficiency causes rickets.
Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation.
We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence.
Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by in vivo bioluminescence.
As the result, strong activity for ossification was observed with 1α,25-hydroxyvitamin D3.
Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.
American Psychological Association (APA)
Nakanishi, Tomoko& Saito, Rumiko& Taniguchi, Makoto& Oda, Haruka& Soma, Atsumi& Yasunaga, Mayu…[et al.]. 2013. In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031055
Modern Language Association (MLA)
Nakanishi, Tomoko…[et al.]. In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031055
American Medical Association (AMA)
Nakanishi, Tomoko& Saito, Rumiko& Taniguchi, Makoto& Oda, Haruka& Soma, Atsumi& Yasunaga, Mayu…[et al.]. In Vivo Determination of Vitamin D Function Using Transgenic Mice Carrying a Human Osteocalcin Luciferase Reporter Gene. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031055