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ScMT2-1-3, a Metallothionein Gene of Sugarcane, Plays an Important Role in the Regulation of Heavy Metal ToleranceAccumulation
Joint Authors
You-Xiong, Que
Guo, Jinlong
Su, Yachun
Wang, Hengbo
Gao, Shiwu
Xu, Jingsheng
Li-Ping, Xu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Plant metallothioneins (MTs), which are cysteine-rich, low-molecular-weight, and metal-binding proteins, play important roles in detoxification, metal ion homeostasis, and metal transport adjustment.
In this study, a novel metallothionein gene, designated as ScMT2-1-3 (GenBank Accession number JQ627644), was identified from sugarcane.
ScMT2-1-3 was 700 bp long, including a 240 bp open reading frame (ORF) encoding 79 amino acid residues.
A His-tagged ScMT2-1-3 protein was successfully expressed in Escherichia coli system which had increased the host cell’s tolerance to Cd2+, Cu2+, PEG, and NaCl.
The expression of ScMT2-1-3 was upregulated under Cu2+ stress but downregulated under Cd2+ stress.
Real-time qPCR demonstrated that the expression levels of ScMT2-1-3 in bud and root were over 14 times higher than those in stem and leaf, respectively.
Thus, both the E.
coli assay and sugarcane plantlets assay suggested that ScMT2-1-3 is significantly involved in the copper detoxification and storage in the cell, but its functional mechanism in cadmium detoxification and storage in sugarcane cells needs more testification though its expressed protein could obviously increase the host E.
coli cell’s tolerance to Cd2+.
ScMT2-1-3 constitutes thus a new interesting candidate for elucidating the molecular mechanisms of MTs-implied plant heavy metal tolerance/accumulation and for developing sugarcane phytoremediator varieties.
American Psychological Association (APA)
Guo, Jinlong& Li-Ping, Xu& Su, Yachun& Wang, Hengbo& Gao, Shiwu& Xu, Jingsheng…[et al.]. 2013. ScMT2-1-3, a Metallothionein Gene of Sugarcane, Plays an Important Role in the Regulation of Heavy Metal ToleranceAccumulation. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031067
Modern Language Association (MLA)
Guo, Jinlong…[et al.]. ScMT2-1-3, a Metallothionein Gene of Sugarcane, Plays an Important Role in the Regulation of Heavy Metal ToleranceAccumulation. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1031067
American Medical Association (AMA)
Guo, Jinlong& Li-Ping, Xu& Su, Yachun& Wang, Hengbo& Gao, Shiwu& Xu, Jingsheng…[et al.]. ScMT2-1-3, a Metallothionein Gene of Sugarcane, Plays an Important Role in the Regulation of Heavy Metal ToleranceAccumulation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031067
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031067