Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens
Joint Authors
Talamás-Rohana, Patricia
Hernández-Ramírez, V. I.
Herrera-Martínez, M.
Lagunes-Guillén, A. E.
Chávez-Munguía, Bibiana
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In the genus Entamoeba, actin reorganization is necessary for cyst differentiation; however, its role is still unknown.
The aim of this work was to investigate the role of actin and encystation-related proteins during Entamoeba invadens encystation.
Studied proteins were actin, RhoA, a small GTPase involved through its effectors in the rearrangement of the actin cytoskeleton; Rab11, a protein involved in the transport of encystation vesicles; and enolase, as an encystment vesicles marker.
Results showed a high level of polymerized actin accompanied by increased levels of RhoA-GTP during cell rounding and loss of vacuoles.
Cytochalasin D, an actin polymerization inhibitor, and Y27632, an inhibitor of RhoA activity, reduced encystment in 80%.
These inhibitors also blocked cell rounding, disposal of vacuoles, and the proper formation of the cysts wall.
At later times, F-actin and Rab11 colocalized with enolase, suggesting that Rab11 could participate in the transport of the cyst wall components through the F-actin cytoskeleton.
These results suggest that actin cytoskeleton rearrangement is playing a decisive role in determining cell morphology changes and helping with the transport of cell wall components to the cell surface during encystment of E.
invadens.
American Psychological Association (APA)
Herrera-Martínez, M.& Hernández-Ramírez, V. I.& Lagunes-Guillén, A. E.& Chávez-Munguía, Bibiana& Talamás-Rohana, Patricia. 2013. Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1031093
Modern Language Association (MLA)
Herrera-Martínez, M.…[et al.]. Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1031093
American Medical Association (AMA)
Herrera-Martínez, M.& Hernández-Ramírez, V. I.& Lagunes-Guillén, A. E.& Chávez-Munguía, Bibiana& Talamás-Rohana, Patricia. Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1031093
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031093