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Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PLA2 Bleu TX-III
Joint Authors
Marangoni, Fábio André
Landucci, Elen Cristina Teizem
Ponce Soto, L. A.
Marangoni, Sérgio
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Bleu TX-III was isolated from Bothrops leucurus snake venom on one-step analytical chromatography reverse phase HPLC, was homogeneous on SDS-PAGE, and was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry in 14243.8 Da.
Multiple alignments of Bleu TX-III show high degree of homology with basic PLA2 myotoxins from other Bothrops venoms.
Our studies on local and systemic myotoxicity “in vivo” reveal that Bleu TX-III is myotoxin with local but not systemic action due to the decrease in the plasmatic CK levels when Bleu TX-III is administrated by intravenous route in mice (dose 1 and 5 μg).
And at a dose of 20 μg myotoxin behaves like a local and systemic action.
Bleu TX-III induced moderate marked paw edema, evidencing the local increase in vascular permeability.
The inflammatory events induced in the mice (I.M.) were investigated.
The increase in the levels of IL-1, IL-6, and TNF-α was observed in the plasma.
It is concluded that Bleu TX-III induces inflammatory events in this model.
The enzymatic phospholipid hydrolysis may be relevant to these phenomena.
Bothrops leucurus venom is still not extensively explored, and the knowledge of its toxins separately through the study of structure/function will contribute for a better understanding of its action mechanism.
American Psychological Association (APA)
Marangoni, Fábio André& Ponce Soto, L. A.& Marangoni, Sérgio& Landucci, Elen Cristina Teizem. 2013. Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PLA2 Bleu TX-III. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031125
Modern Language Association (MLA)
Marangoni, Fábio André…[et al.]. Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PLA2 Bleu TX-III. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031125
American Medical Association (AMA)
Marangoni, Fábio André& Ponce Soto, L. A.& Marangoni, Sérgio& Landucci, Elen Cristina Teizem. Unmasking Snake Venom of Bothrops leucurus: Purification and Pharmacological and Structural Characterization of New PLA2 Bleu TX-III. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031125
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031125