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Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types
Joint Authors
Hwang, Sang Mee
Kim, Taek Soo
Park, Kyoung Un
Hong, Yun Ji
Song, Eun Young
Kim, Mi Jung
Chang, Ho Eun
Song, Junghan
Han, Kyou-Sup
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109.
We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF).
HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis.
Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products.
CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB.
However, the increase of expression level varied among the PBSC and granulocyte products.
The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected.
HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.
American Psychological Association (APA)
Hwang, Sang Mee& Kim, Mi Jung& Chang, Ho Eun& Hong, Yun Ji& Kim, Taek Soo& Song, Eun Young…[et al.]. 2013. Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1031133
Modern Language Association (MLA)
Hwang, Sang Mee…[et al.]. Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1031133
American Medical Association (AMA)
Hwang, Sang Mee& Kim, Mi Jung& Chang, Ho Eun& Hong, Yun Ji& Kim, Taek Soo& Song, Eun Young…[et al.]. Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1031133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031133