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Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract
Joint Authors
Sameshima, Hiroshi
Harishkumar, Madhyastha
Masatoshi, Yamaguchi
Masugi, Maruyama
Ikenoue, Tsuyomu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In the present investigation, we examined the effect of Hyuganatsu (Citrus tamurana) extract (HE) on skin fibroblast (TIG-119) proliferation and migration during in vitro wound healing.
HE selectively inhibited proliferation of TIG-119 cells at higher concentration (>1.0 mg/mL); at lower concentrations (0.1, 0.25, 0.5, and 0.75 mg/mL), it exhibited linear and time-dependent cell proliferation.
In vitro scratch wound healing studies showed that the HE also accelerated the migration of cells towards the wounded region.
Cytometric analysis demonstrated that HE extract did not alter G1/0 and S phases of cell cycle in any concentration studied; however, G2/M phases of cell cycle were significantly (P<0.05) accelerated at 0.75 mg/mL dose.
RT-PCR and Western blotting analysis indicated that HE markedly overexpressed levels of Rac-1, Rho-A, and Cdc-42 mRNA and the respective proteins.
Cyclin-dependent kinases (Cdk-1 and -2) gene expression activity was significantly (P<0.05) increased, but protein content decreased during treatment with HE.
The induction of Cdk-1 and -2 by HE was abolished by inhibitors, transcription (DRB), and translation (CHX), implying transcriptional regulation that required de novo protein synthesis.
American Psychological Association (APA)
Harishkumar, Madhyastha& Masatoshi, Yamaguchi& Sameshima, Hiroshi& Ikenoue, Tsuyomu& Masugi, Maruyama. 2013. Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1031152
Modern Language Association (MLA)
Harishkumar, Madhyastha…[et al.]. Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1031152
American Medical Association (AMA)
Harishkumar, Madhyastha& Masatoshi, Yamaguchi& Sameshima, Hiroshi& Ikenoue, Tsuyomu& Masugi, Maruyama. Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1031152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031152