Characterization of CD8+ T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL)
Joint Authors
Neyns, Bart
Benteyn, Daphné
Van Nuffel, An M. T.
Corthals, Jurgen
Heirman, Carlo
Thielemans, Kris
Bonehill, Aude
Wilgenhof, Sofie
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Treatment of melanoma patients with mRNA electroporated dendritic cells (TriMixDC-MEL) stimulates T-cell responses against the presented tumor-associated antigens (TAAs).
In the current clinical trials, melanoma patients with systemic metastases are treated, requiring priming and/or expansion of preexisting TAA-specific T cells that are able to migrate to both the skin and internal organs.
We monitored the presence of TAA-specific CD8+ T cells infiltrating the skin at sites of intradermal TriMixDC-MEL injection (SKILs) and within the circulation of melanoma patients treated in two clinical trials.
In 10 out of fourteen (71%) patients screened, CD8+ T cells recognizing any of the four TAA presented by TriMixDC-MEL cellular vaccine were found in both compartments.
In total, 30 TAA-specific T-cell responses were detected among the SKILs and 29 among peripheral blood T cells, of which 24 in common.
A detailed characterization of the antigen specificity of CD8+ T-cell populations in four patients indicates that the majority of the epitopes detected were only recognized by CD8+ T cells derived from either skin biopsies or peripheral blood, indicating that some compartmentalization occurs after TriMix-DC therapy.
To conclude, functional TAA-specific CD8+ T cells distribute both to the skin and peripheral blood of patients after TriMixDC-MEL therapy.
American Psychological Association (APA)
Benteyn, Daphné& Van Nuffel, An M. T.& Wilgenhof, Sofie& Corthals, Jurgen& Heirman, Carlo& Neyns, Bart…[et al.]. 2013. Characterization of CD8+ T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL). BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1031161
Modern Language Association (MLA)
Benteyn, Daphné…[et al.]. Characterization of CD8+ T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL). BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1031161
American Medical Association (AMA)
Benteyn, Daphné& Van Nuffel, An M. T.& Wilgenhof, Sofie& Corthals, Jurgen& Heirman, Carlo& Neyns, Bart…[et al.]. Characterization of CD8+ T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL). BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1031161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031161