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Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults
Joint Authors
Radhakrishnan, Ammu
Raj, Vijaya Lechimi
Tan, Lee-Keng
Liam, Chong-Kin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Asthma susceptibility genes are mapped to a region on human chromosome 5q31-q33, which contains a cluster of proinflammatory cytokine genes such as interleukin-13 (IL-13), which is associated with asthma.
This study investigated the allele frequencies of two single nucleotide polymorphisms (SNPs) (−1111C>T and 4257C>A) in the IL-13 gene between asthmatics and healthy volunteers as well as the relationship between these SNPs and IL-13 production.
DNA extracted from buffy coat of asthmatic and control subjects was genotyped using the PCR-RFLP method.
Amount of IL-13 produced by mitogen-stimulated peripheral blood leucocytes PBLs (PBLs) was determined by ELISA.
The frequencies of the −1111C and 4257G wild-type alleles were 0.52 and 0.55 in asthmatics and were 0.67 and 0.56 in controls.
A significant (P<0.05) association was found between genotype and allele frequencies of SNP at position −1111C>T between asthmatic and control groups (OR, 1.810; 95% CI = 1.184 to 2.767; P<0.05).
The mitogen-stimulated PBLs from asthmatics produced higher amounts of IL-13 production (P<0.001).
The 4257GA heterozygous and 4257AA homozygous mutant alleles were associated with higher IL-13 production in asthmatics (P<0.05).
Our results show that the −1111T mutant allele are associated with asthma and the 4257A mutant alleles are associated with elevated IL-13 production.
American Psychological Association (APA)
Radhakrishnan, Ammu& Raj, Vijaya Lechimi& Tan, Lee-Keng& Liam, Chong-Kin. 2013. Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1031165
Modern Language Association (MLA)
Radhakrishnan, Ammu…[et al.]. Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1031165
American Medical Association (AMA)
Radhakrishnan, Ammu& Raj, Vijaya Lechimi& Tan, Lee-Keng& Liam, Chong-Kin. Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1031165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031165