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CD90- (Thy-1-) High Selection Enhances Reprogramming Capacity of Murine Adipose-Derived Mesenchymal Stem Cells
Joint Authors
Kawamoto, Koichi
Konno, Masamitsu
Nagano, Hiroaki
Nishikawa, Shimpei
Tomimaru, Yoshito
Akita, Hirofumi
Hama, Naoki
Wada, Hiroshi
Kobayashi, Shogo
Eguchi, Hidetoshi
Tanemura, Masahiro
Ito, Toshinori
Doki, Yuichiro
Ishii, Hideshi
Mori, Masaki
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Mesenchymal stem cells (MSCs), including adipose tissue-derived mesenchymal stem cells (ADSC), are multipotent and can differentiate into various cell types possessing unique immunomodulatory features.
Several clinical trials have demonstrated the safety and possible efficacy of MSCs in organ transplantation.
Thus, stem cell therapy is promising for tolerance induction.
In this study, we assessed the reprogramming capacity of murine ADSCs and found that CD90 (Thy-1), originally discovered as a thymocyte antigen, could be a useful marker for cell therapy.
Method.
Murine ADSCs were isolated from B6 mice, sorted using a FACSAria cell sorter by selection of CD90Hi or CD90Lo, and then transduced with four standard factors (4F; Oct4, Sox2, Klf4, and c-Myc).
Results.
Unsorted, CD90Hi-sorted, and CD90Lo-sorted murine ADSCs were reprogrammed using standard 4F transduction.
CD90Hi ADSCs showed increased numbers of alkaline phosphatase-positive colonies compared with CD90Lo ADSCs.
The relative reprogramming efficiencies of unsorted, CD90Hi-sorted, and CD90Lo-sorted ADSCs were 100%, 116.5%, and 74.7%, respectively.
CD90Hi cells were more responsive to reprogramming.
Conclusion.
CD90Hi ADSCs had greater reprogramming capacity than CD90Lo ADSCs, suggesting that ADSCs have heterogeneous subpopulations.
Thus, CD90Hi selection presents an effective strategy to isolate a highly suppressive subpopulation for stem cell-based tolerance induction therapy.
American Psychological Association (APA)
Kawamoto, Koichi& Konno, Masamitsu& Nagano, Hiroaki& Nishikawa, Shimpei& Tomimaru, Yoshito& Akita, Hirofumi…[et al.]. 2013. CD90- (Thy-1-) High Selection Enhances Reprogramming Capacity of Murine Adipose-Derived Mesenchymal Stem Cells. Disease Markers،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1031198
Modern Language Association (MLA)
Kawamoto, Koichi…[et al.]. CD90- (Thy-1-) High Selection Enhances Reprogramming Capacity of Murine Adipose-Derived Mesenchymal Stem Cells. Disease Markers No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1031198
American Medical Association (AMA)
Kawamoto, Koichi& Konno, Masamitsu& Nagano, Hiroaki& Nishikawa, Shimpei& Tomimaru, Yoshito& Akita, Hirofumi…[et al.]. CD90- (Thy-1-) High Selection Enhances Reprogramming Capacity of Murine Adipose-Derived Mesenchymal Stem Cells. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1031198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031198