mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study
Joint Authors
Shivaprasad, B. M.
Padmavati, P.
Savita, S.
Rithesh, K.
Kripal, Krishna
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background and Objectives.
Matrix metalloproteinases degrade extracellular membrane and also release bioactive fragments and growth factors, thus influencing fundamental biological and pathological processes.
Epilysin (MMP-28) differs from most other MMPs as it is expressed in a number of normal tissues, suggestive of functions in tissue homeostasis.
The aim of the present study was to quantitatively evaluate and compare the mRNA expression of epilysin (MMP-28) in gingival tissues of healthy patients and of patients affected by chronic or aggressive periodontitis.
Methods.
A total of 60 subjects, 20 periodontally healthy subjects, 20 with chronic periodontitis, and 20 with aggressive periodontitis, were included in this study.
Periodontal status was evaluated by measuring gingival index, probing depth and clinical attachment level.
mRNA expression of MMP-28 was determined by quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in gingival tissue samples collected.
Results.
Relative quantification of mRNA expression of MMP-28 was highest in healthy tissues (RQ=0.97) when compared to subjects with chronic periodontitis (RQ=0.37) and aggressive periodontitis (RQ=0.23), but the difference was not statistically significant.
Conclusion.
mRNA expression of MMP-28 was highest in healthy tissues when compared to diseased periodontal tissues suggesting that MMP-28 could act as a biomarker for periodontal health.
American Psychological Association (APA)
Padmavati, P.& Savita, S.& Shivaprasad, B. M.& Kripal, Krishna& Rithesh, K.. 2013. mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study. Disease Markers،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031214
Modern Language Association (MLA)
Padmavati, P.…[et al.]. mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study. Disease Markers No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031214
American Medical Association (AMA)
Padmavati, P.& Savita, S.& Shivaprasad, B. M.& Kripal, Krishna& Rithesh, K.. mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study. Disease Markers. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031214