Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages
Joint Authors
Meng, Tao
Wang, Shuqin
Zhang, Xinyu
Yu, Jingya
Yu, Jingui
Bo, Qiyu
Ma, Zhiyong
Lei, Zhen
Wu, Jianbo
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
During an infection, lipopolysaccharide (LPS) stimulates the production of reactive oxygen species (ROS), which is mediated, in large part, by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs); NOX2 is the major NOX isoform found in the macrophage cell membrane.
While the immunomodulatory activity of propofol is highly documented, its effect on the LPS-induced NOX2/ROS/NF-κB signaling pathway in macrophages has not been addressed.
In present study, we used murine macrophage cell line RAW264.7 pretreated with propofol and stimulated with LPS.
IL-6 and TNF-α expression, ROS production, and NOX activity were determined.
Results showed that propofol attenuated LPS-induced TNF-α and IL-6 expression.
Moreover, LPS-stimulated phosphorylation of NF-κB and generation of ROS were weakened in response to propofol.
Propofol also reduced LPS-induced NOX activity and expression of gp91phox and p47phox.
We conclude that propofol modulates LPS signaling in macrophages by reducing NOX-mediated production of TNF-α and IL-6.
American Psychological Association (APA)
Meng, Tao& Yu, Jingya& Lei, Zhen& Wu, Jianbo& Wang, Shuqin& Bo, Qiyu…[et al.]. 2013. Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031274
Modern Language Association (MLA)
Meng, Tao…[et al.]. Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages. Journal of Immunology Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1031274
American Medical Association (AMA)
Meng, Tao& Yu, Jingya& Lei, Zhen& Wu, Jianbo& Wang, Shuqin& Bo, Qiyu…[et al.]. Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1031274
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031274