Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients
Joint Authors
Zhou, Qiao
Shi, Guixiu
Zhou, Bin
Zhang, Jing
Long, Li
Wu, Tong
Source
Journal of Immunology Research
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-10
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Objective.
To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP).
Materials and Methods.
DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay.
Results.
miR-124-a1 and miR-124-a2 promoter methylation were both seen in 71.4% of RA samples compared to 16.7% of OA samples.
miR-124-a3 promoter methylation was seen in 57.1% of RA samples and 0% of OA samples.
All the three loci were unmethylated in 3 healthy controls.
Conclusion.
The methylation status of miR-124a seen in this study concurs with that reported in tumor cells, indicating epigenetic dysregulation constituents, a mechanism in the development of rheumatoid arthritis.
American Psychological Association (APA)
Zhou, Qiao& Long, Li& Shi, Guixiu& Zhang, Jing& Wu, Tong& Zhou, Bin. 2013. Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031284
Modern Language Association (MLA)
Zhou, Bin…[et al.]. Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients. Journal of Immunology Research No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1031284
American Medical Association (AMA)
Zhou, Qiao& Long, Li& Shi, Guixiu& Zhang, Jing& Wu, Tong& Zhou, Bin. Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1031284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031284