β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro
Joint Authors
Nakagawa, Yusuke
Muneta, Takeshi
Ichinose, Shizuko
Hakamatsuka, Yasuharu
Koga, Hideyuki
Sekiya, Ichiro
Tsuji, Kunikazu
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
β-Tricalcium phosphate (β-TCP) micron particles whose diameters range from 1 μm to 10 μm have been recently developed, however, their biological effects remain unknown.
We investigated the biological effects of β-TCP micron particles on proliferation, cytotoxicity, and calcification of human synovial mesenchymal stem cells (MSCs).
MSCs were cultured without dexamethasone, β-glycerophosphate, or ascorbic acid.
1.0 mg/mL β-TCP micron particles inhibited proliferation of MSCs significantly and increased dead cells.
In the contact condition, 0.1 mg/mL β-TCP micron particles promoted calcification of MSCs evaluated by alizarin red staining and enhanced mRNA expressions of runx2, osteopontin, and type I collagen.
In the noncontact condition, these effects were not observed.
0.1 mg/mL β-TCP micron particles increased calcium concentration in the medium in the contact condition, while 1.0 mg/mL β-TCP micron particles decreased calcium and phosphorus concentrations in the medium in the noncontact condition.
By transmission electron microscopy, β-TCP micron particles were localized in the phagosome of MSCs and were dissolved.
In conclusion, β-TCP micron particles promoted calcification of MSCs and enhanced osteogenesis-related gene expressions in vitro.
American Psychological Association (APA)
Nakagawa, Yusuke& Muneta, Takeshi& Tsuji, Kunikazu& Ichinose, Shizuko& Hakamatsuka, Yasuharu& Koga, Hideyuki…[et al.]. 2013. β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1031380
Modern Language Association (MLA)
Nakagawa, Yusuke…[et al.]. β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro. Journal of Nanomaterials No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1031380
American Medical Association (AMA)
Nakagawa, Yusuke& Muneta, Takeshi& Tsuji, Kunikazu& Ichinose, Shizuko& Hakamatsuka, Yasuharu& Koga, Hideyuki…[et al.]. β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1031380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031380