Detection of Carbofuran with Immobilized Acetylcholinesterase Based on Carbon Nanotubes-Chitosan Modified Electrode

Abstract EN

A sensitive and stable enzyme biosensor based on efficient immobilization of acetylcholinesterase (AChE) to MWNTs-modified glassy carbon electrode (GCE) with chitosan (CS) by layer-by-layer (LBL) technique for rapid determination of carbofuran has been devised.

According to the inhibitory effect of carbamate pesticide on the enzymatic activity of AChE, we use carbofuran as a model pesticide.

The inhibitory effect of carbofuran on the biosensor was proportional to concentration of carbofuran in the range from 10−10 g/L to 10−3 g/L with a detection limit of 10−12 g/L.

This biosensor is a promising new method for pesticide analysis.