In Vivo Study of Ligament-Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold
Joint Authors
Zhou, Chunyan
Ao, Yingfang
Wang, Xiaokun
Zhang, Xin
Zhu, Jingxian
Shao, Zhenxing
Dai, Linghui
Li, La
Hu, Xiaoqing
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Electrospinning nanofibrous scaffold was commonly used in tissue regeneration recently.
Nanofibers with specific topological characteristics were reported to be able to induce osteogenic differentiation of MSCs.
In this in vivo study, autologous tendon grafts with lattice-like nanofibrous scaffold wrapping at two ends of autologous tendon were used to promote early stage of ligament-bone healing after rabbit ACL reconstruction.
To utilize native MSCs from bone marrow, an MSCs specific affinity peptide E7 was conjugated to nanofibrous meshes.
After 3 months, H-E assessment and specific staining of collagen type I, II, and III showed direct ligament-bone insertion with typical four zones (bone, calcified fibrocartilage, fibrocartilage, and ligament) in bioactive scaffold reconstruction group.
Diameters of bone tunnel were smaller in nanofibrous scaffold conjugated E7 peptide group than those in control group.
The failure load of substitution complex also indicated a stronger ligament-bone insertion healing using bioactive scaffold.
In conclusion, lattice-like nanofibrous scaffold with specific MSCs affinity peptide has great potential in promoting early stage of ligament-bone healing after ACL reconstruction.
American Psychological Association (APA)
Zhu, Jingxian& Zhang, Xin& Shao, Zhenxing& Dai, Linghui& Li, La& Hu, Xiaoqing…[et al.]. 2013. In Vivo Study of Ligament-Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031592
Modern Language Association (MLA)
Zhu, Jingxian…[et al.]. In Vivo Study of Ligament-Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold. Journal of Nanomaterials No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1031592
American Medical Association (AMA)
Zhu, Jingxian& Zhang, Xin& Shao, Zhenxing& Dai, Linghui& Li, La& Hu, Xiaoqing…[et al.]. In Vivo Study of Ligament-Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1031592