Kisspeptin-10 Modulates the Proliferation and Differentiation of the Rhesus Monkey Derived Stem Cell Line: R366.4
Joint Authors
Huma, Tanzeel
Wang, Zhengbo
Rizak, Joshua
Ahmad, Fiaz
Shahab, Muhammad
Ma, YuanYe
Yang, Shangchuan
Hu, Xintian
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The rhesus monkey embryonic stem cell line R366.4 has been identified to differentiate into a number of cell types.
However, it has not been well characterized for its response to drugs affecting reproductive endocrinology.
Kisspeptins (KPs) are ligands for the GPR-54, which is known to modulate reproductive function.
The current study was designed to determine the effect of the KP-10 peptide on R366.4 cells and to investigate the role of KP-GPR54 in the cell proliferation process.
Four different doses (0.1, 1, 10, and 100 nM) of KP-10 and control were selected to evaluate cell growth parameters and cellular morphological changes over a 72 hr period.
The cells were treated with kisspeptin-10 during the early rosette stage.
Proliferation rates, analyzed by flow cytometry and cell count methods, were found to be decreased after treatment.
Moreover, the number of rosettes was found to decrease following KP-10 treatments.
Morphological changes consisting of neuronal projections were also witnessed.
This suggested that KP-10 had an antiproliferative effect on R366.4 cells leading to a differentiation state and morphological changes consistent with neuronal stem cell development.
The R366.4 stem cell line differentiates based on kisspeptin signaling and may be used to investigate reproductive cell endocrinology in vitro.
American Psychological Association (APA)
Huma, Tanzeel& Wang, Zhengbo& Rizak, Joshua& Ahmad, Fiaz& Shahab, Muhammad& Ma, YuanYe…[et al.]. 2013. Kisspeptin-10 Modulates the Proliferation and Differentiation of the Rhesus Monkey Derived Stem Cell Line: R366.4. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032563
Modern Language Association (MLA)
Huma, Tanzeel…[et al.]. Kisspeptin-10 Modulates the Proliferation and Differentiation of the Rhesus Monkey Derived Stem Cell Line: R366.4. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1032563
American Medical Association (AMA)
Huma, Tanzeel& Wang, Zhengbo& Rizak, Joshua& Ahmad, Fiaz& Shahab, Muhammad& Ma, YuanYe…[et al.]. Kisspeptin-10 Modulates the Proliferation and Differentiation of the Rhesus Monkey Derived Stem Cell Line: R366.4. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032563
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032563