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Essential Roles of PKA, iNOS, CD95CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents
Joint Authors
Cunha, Fernando Queiroz
Arruda, Maria Augusta
Queto, T.
Aranha, M. G.
De Luca, B.
Luz, R. A.
Lopes, R. S.
Gaspar-Elsas, M. I.
Barradas, Mônica
Cunha, Thiago Mattar
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Up- and downregulation of eosinopoiesis control pulmonary eosinophilia in human asthma.
In mice, eosinopoiesis is suppressed in vitro by prostaglandin E2 (PGE2) and in vivo by diethylcarbamazine, through a proapoptotic mechanism sequentially requiring inducible NO synthase (iNOS) and the ligand for death receptor CD95 (CD95L).
We examined the roles of iNOS, cAMP-mediated signaling, caspases, and CD95L/CD95 in suppression of eosinopoiesis by PGE2 and other agents signaling through cAMP.
Bone-marrow collected from BALB/c mice, or from iNOS-, CD95-, or CD95L-deficient mutants (and wild-type controls), was cultured with interleukin-5 (IL-5), alone or associated with PGE2, cAMP-inducing/mimetic agents, caspase inhibitor zVAD-fmk, iNOS inhibitor aminoguanidine, or combinations thereof, and eosinopoiesis was evaluated at various times.
PGE2, added up to 24 hours of culture, dose-dependently suppressed eosinopoiesis, by inducing apoptosis.
This effect was (a) paralleled by induction of iNOS in eosinophils; (b) duplicated by sodium nitroprusside, isoproterenol, and cAMP-inducing/mimetic agents; (c) prevented by protein kinase A inhibition.
NO was produced through iNOS by dibutyryl-cAMP-stimulated bone-marrow.
Overall, PGE2 and isoproterenol shared a requirement for four effector elements (iNOS, CD95L, CD95, and terminal caspases), which together define a pathway targeted by several soluble up- and downmodulators of eosinopoiesis, including drugs, mediators of inflammation, and cytokines.
American Psychological Association (APA)
De Luca, B.& Lopes, R. S.& Barradas, Mônica& Luz, R. A.& Queto, T.& Aranha, M. G.…[et al.]. 2013. Essential Roles of PKA, iNOS, CD95CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032659
Modern Language Association (MLA)
De Luca, B.…[et al.]. Essential Roles of PKA, iNOS, CD95CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents. The Scientific World Journal No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1032659
American Medical Association (AMA)
De Luca, B.& Lopes, R. S.& Barradas, Mônica& Luz, R. A.& Queto, T.& Aranha, M. G.…[et al.]. Essential Roles of PKA, iNOS, CD95CD95L, and Terminal Caspases in Suppression of Eosinopoiesis by PGE2 and Other cAMP-Elevating Agents. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1032659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032659