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Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C636 Cells
Joint Authors
Farias, Kleber Juvenal Silva
Machado, Paula Renata Lima
da Fonseca, Benedito Antônio Lopes
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world.
Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue.
Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2).
Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment.
Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells.
In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU).
These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.
American Psychological Association (APA)
Farias, Kleber Juvenal Silva& Machado, Paula Renata Lima& da Fonseca, Benedito Antônio Lopes. 2013. Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C636 Cells. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1032742
Modern Language Association (MLA)
Farias, Kleber Juvenal Silva…[et al.]. Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C636 Cells. The Scientific World Journal No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1032742
American Medical Association (AMA)
Farias, Kleber Juvenal Silva& Machado, Paula Renata Lima& da Fonseca, Benedito Antônio Lopes. Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C636 Cells. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1032742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032742