Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α
Joint Authors
Liu, Bo
Gong, Xin
Chang, Shaohong
Sun, Peng
Wu, Jun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Nα-terminal acetylation of peptides plays an important biological role but is rarely observed in prokaryotes.
Nα-terminal acetylated thymosin α1 (Tα1), a 28-amino-acid peptide, is an immune modifier that has been used in the clinic to treat hepatitis B and C virus (HBV/HCV) infections.
We previously documented Nα-terminal acetylation of recombinant prothymosin α (ProTα) in E.
coli.
Here we present a method for production of Nα-acetylated Tα1 from recombinant ProTα.
The recombinant ProTα was cleaved by human legumain expressed in Pichia pastoris to release Tα1 in vitro.
The Nα-acetylated Tα1 peptide was subsequently purified by reverse phase and cation exchange chromatography.
Mass spectrometry indicated that the molecular mass of recombinant Nα-acetylated Tα1 was 3108.79 in, which is identical to the mass of Nα-acetylated Tα1 produced by total chemical synthesis.
This mass corresponded to the nonacetylated Tα1 mass with a 42 Da increment.
The retention time of recombinant Nα-acetylated Tα1 and chemosynthetic Nα-acetylated Tα1 were both 15.4 min in RP-high performance liquid chromatography (HPLC).
These data support the use of an E.
coli expression system for the production of recombinant human Nα-acetylated Tα1 and also will provide the basis for the preparation of recombinant acetylated peptides in E.
coli.
American Psychological Association (APA)
Liu, Bo& Gong, Xin& Chang, Shaohong& Sun, Peng& Wu, Jun. 2013. Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1032857
Modern Language Association (MLA)
Liu, Bo…[et al.]. Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1032857
American Medical Association (AMA)
Liu, Bo& Gong, Xin& Chang, Shaohong& Sun, Peng& Wu, Jun. Generation of Mature Nα-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1032857
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032857