Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
Joint Authors
Orts, William J.
Tabassum, Romana
Majeed, Tanveer
Lee, Charles C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis.
The enzyme was determined to be a serine protease based on inhibition by PMSF.
Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10).
In addition, the enzyme had an elevated optimum temperature (60°C).
The protease was also stable in the presence of many surfactants and oxidant.
Thus, the C.
proteolyticus protease has potential applications in industries such as the detergent market.
American Psychological Association (APA)
Majeed, Tanveer& Tabassum, Romana& Orts, William J.& Lee, Charles C.. 2013. Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1032869
Modern Language Association (MLA)
Majeed, Tanveer…[et al.]. Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease. The Scientific World Journal No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1032869
American Medical Association (AMA)
Majeed, Tanveer& Tabassum, Romana& Orts, William J.& Lee, Charles C.. Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1032869
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032869
