Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice
Joint Authors
Rerksiri, Wirat
Zhang, Xianwen
Xiong, Hairong
Chen, Xinbo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
During the long evolutionary process, plant gradually formed a series of strategies and mechanisms to cope with stress environment such as drought, heat, cold, and high salinity.
Six highly heat responsive genes were identified in rice by microarray data analysis.
The qRT-PCR analysis confirmed that the expression of these six genes were highly heat inducible and moderately responded to salt stress, polyethylene glycol, and abscisic acid treatment, but little affected by cold treatment.
Promoters of the three highly heat-inducible genes (OsHsfB2cp, PM19p, and Hsp90p) were used to drive GUS gene expression in rice.
The results of the GUS gene expression, histochemical staining, and GUS activities in panicles and flag leaves of the transgenic rice plants confirmed high heat-induced GUS activities and moderate drought-induced activities.
The three promoters exhibited similar high activity lever in rice leaf under heat, but OsHsfB2cp and PM19p showed much higher activities in panicles under heat stress.
Our work confirmed that the OsHsfB2c and PM19 promoters were highly heat inducible and further characterization and reconstruction of cis-elements in their promoters could lead to the development of highly effective heat-inducible promoters for plant genetic engineering.
American Psychological Association (APA)
Rerksiri, Wirat& Zhang, Xianwen& Xiong, Hairong& Chen, Xinbo. 2013. Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1032872
Modern Language Association (MLA)
Rerksiri, Wirat…[et al.]. Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1032872
American Medical Association (AMA)
Rerksiri, Wirat& Zhang, Xianwen& Xiong, Hairong& Chen, Xinbo. Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1032872
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032872