Nucleotide Sequencing and Identification of Some Wild Mushrooms
Joint Authors
Mandal, Aninda
Das, Sudip Kumar
Gupta, Sudha
Paul, Rita
Saha, Aditi
Sengupta, Sonali
Dubey, Priyanka Kumari
Datta, Animesh K.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned.
The sequences were aligned using ClustalW software program.
The aligned sequences revealed identity (homology percentage from GenBank data base) of Amanita hemibapha [CN (Chota Nagpur) 1, % identity 99 (JX844716.1)], Amanita sp.
[CN 2, % identity 98 (JX844763.1)], Astraeus hygrometricus [CN 3, % identity 87 (FJ536664.1)], Termitomyces sp.
[CN 4, % identity 90 (JF746992.1)], Termitomyces sp.
[CN 5, % identity 99 (GU001667.1)], T.
microcarpus [CN 6, % identity 82 (EF421077.1)], Termitomyces sp.
[CN 7, % identity 76 (JF746993.1)], and Volvariella volvacea [CN 8, % identity 100 (JN086680.1)].
Although out of 8 mushrooms 4 could be identified up to species level, the nucleotide sequences of the rest may be relevant to further characterization.
A phylogenetic tree is constructed using Neighbor-Joining method showing interrelationship between/among the mushrooms.
The determined nucleotide sequences of the mushrooms may provide additional information enriching GenBank database aiding to molecular taxonomy and facilitating its domestication and characterization for human benefits.
American Psychological Association (APA)
Das, Sudip Kumar& Mandal, Aninda& Datta, Animesh K.& Gupta, Sudha& Paul, Rita& Saha, Aditi…[et al.]. 2013. Nucleotide Sequencing and Identification of Some Wild Mushrooms. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032880
Modern Language Association (MLA)
Das, Sudip Kumar…[et al.]. Nucleotide Sequencing and Identification of Some Wild Mushrooms. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1032880
American Medical Association (AMA)
Das, Sudip Kumar& Mandal, Aninda& Datta, Animesh K.& Gupta, Sudha& Paul, Rita& Saha, Aditi…[et al.]. Nucleotide Sequencing and Identification of Some Wild Mushrooms. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032880
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032880