Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.)
Joint Authors
Kim, Jonggeun
Choi, Bosung
Park, Young-Hwan
Cho, Byoung-Kwan
Lim, Hyoun-Sub
Natarajan, Savithiry
Bae, Hanhong
Park, Sang Un
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.).
Kenaf is a fast-growing dicotyledonous plant valued for its biomass.
F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis.
The full length of the F5H ortholog was cloned and characterized.
The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF) encoding 518 amino acids (GenBank Accession number JX524278).
The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78%) with that of Populus trichocarpa.
Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses.
The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old) of stem tissues, with a certain level of expression in all tissues tested.
The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h), wounding (24 h), cold (24 h), abscisic acid (24 h), and methyl jasmonate (24 h).
American Psychological Association (APA)
Kim, Jonggeun& Choi, Bosung& Park, Young-Hwan& Cho, Byoung-Kwan& Lim, Hyoun-Sub& Natarajan, Savithiry…[et al.]. 2013. Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.). The Scientific World Journal،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1032900
Modern Language Association (MLA)
Kim, Jonggeun…[et al.]. Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.). The Scientific World Journal No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1032900
American Medical Association (AMA)
Kim, Jonggeun& Choi, Bosung& Park, Young-Hwan& Cho, Byoung-Kwan& Lim, Hyoun-Sub& Natarajan, Savithiry…[et al.]. Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.). The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1032900
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032900