A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries
Joint Authors
Pan, Wenting
Mao, Tiantian
Xu, Qing-an
Shao, Jin
Liu, Chang
Fan, Mingwen
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Background.
gcrR gene acts as a negative regulator related to sucrose-dependent adherence in S.
mutans.
It is constructive to test the potential capacity of mutans with gcrR gene deficient in bacteria replacement therapy.
Methods.
In this study, we constructed the mutant by homologous recombination.
The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy.
S.
mutans UA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model.
Results.
Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage.
MS-gcrR-def out-competed S.
mutans UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo.
The former possessed both a lower level of acid production and a stronger colonization potential than S.
mutans UA159.
Conclusion.
These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy.
American Psychological Association (APA)
Pan, Wenting& Mao, Tiantian& Xu, Qing-an& Shao, Jin& Liu, Chang& Fan, Mingwen. 2013. A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032940
Modern Language Association (MLA)
Pan, Wenting…[et al.]. A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1032940
American Medical Association (AMA)
Pan, Wenting& Mao, Tiantian& Xu, Qing-an& Shao, Jin& Liu, Chang& Fan, Mingwen. A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032940
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032940