Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.
Joint Authors
Goel, Nitika
Dubey, Kashyap Kumar
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Present work investigated an apposite and efficient method for extraction of betulinic acid (BA) from the bark of Ziziphus jujubae.
Various extraction methods like stirring extraction, soxhlet extraction, ultrasonic extraction, and microwave assisted extraction (MAE) were evaluated for increasing recovery percentage of BA.
From the raffinate so obtained, BA was isolated.
Thin layer chromatography (TLC) was used to analyze the extract and high performance liquid chromatography (HPLC) for quantification.
The results revealed that the percentage extraction of BA from Z.
jujubae by MAE was more proficient.
As recovery percentage of BA by MAE technique turned out to be maximum, by using response surface methodology (RSM), three process parameters (pH, temperature, and time) were optimized by MAE and it was observed that the optimum parameters (pH 6.5, temp.
70.23°C, and time 3.5 min) gave the maximum recovery of BA (0.44% w/w).
To validate the RSM model, experiments were performed and the highest recovery of BA was found to be 0.4% w/w which is ±0.04% to the predicted value.
Henceforth the extraction efficiency and the substantial saving of time by MAE was more capable than the other extraction techniques.
American Psychological Association (APA)
Dubey, Kashyap Kumar& Goel, Nitika. 2013. Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1032956
Modern Language Association (MLA)
Dubey, Kashyap Kumar& Goel, Nitika. Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1032956
American Medical Association (AMA)
Dubey, Kashyap Kumar& Goel, Nitika. Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1032956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032956