RhoA Regulation of Cardiomyocyte Differentiation
Joint Authors
Crane, Denis I.
Kaarbø, Mari
Murrell, Wayne
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Earlier findings from our laboratory implicated RhoA in heart developmental processes.
To investigate factors that potentially regulate RhoA expression, RhoA gene organisation and promoter activity were analysed.
Comparative analysis indicated strict conservation of both gene organisation and coding sequence of the chick, mouse, and human RhoA genes.
Bioinformatics analysis of the derived promoter region of mouse RhoA identified putative consensus sequence binding sites for several transcription factors involved in heart formation and organogenesis generally.
Using luciferase reporter assays, RhoA promoter activity was shown to increase in mouse-derived P19CL6 cells that were induced to differentiate into cardiomyocytes.
Overexpression of a dominant negative mutant of mouse RhoA (mRhoAN19) blocked this cardiomyocyte differentiation of P19CL6 cells and led to the accumulation of the cardiac transcription factors SRF and GATA4 and the early cardiac marker cardiac α-actin.
Taken together, these findings indicate a fundamental role for RhoA in the differentiation of cardiomyocytes.
American Psychological Association (APA)
Kaarbø, Mari& Crane, Denis I.& Murrell, Wayne. 2013. RhoA Regulation of Cardiomyocyte Differentiation. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1032984
Modern Language Association (MLA)
Kaarbø, Mari…[et al.]. RhoA Regulation of Cardiomyocyte Differentiation. The Scientific World Journal No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1032984
American Medical Association (AMA)
Kaarbø, Mari& Crane, Denis I.& Murrell, Wayne. RhoA Regulation of Cardiomyocyte Differentiation. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1032984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032984