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Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome
Joint Authors
Lee, Yu-Ching
Tsai, Keng-Chang
Wang, Duen-Jen
Liu, Chia-Yu
Leu, Sy-Jye
Yang, Yi-Yuan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
We have previously isolated several IgG rheumatoid factors (RFs) from patients with both rheumatoid arthritis and idiopathic thrombocytopenia purpura using phage display system.
To study IgG RFs in patients with other autoimmune diseases, phage display antibody libraries from a hepatitis C virus infected patient with Sjögren’s syndrome were constructed.
After panning, a specific clone RFL11 was isolated for characterization in advance.
The binding activity and specificity of RFL11 to IgG Fc fragment were comparable to those of RFs previously isolated.
The analysis with existed RF-Fc complex structures indicated the homology model of RFL11 is similar to IgM RF61 complex with high binding affinity of about 6×10-8 M.
This effect resulted from longer complementarity-determining region (CDR) combining key somatic mutations.
In the RFL11-Fc interfaces, the CDR-H3 loop forms a finger-like structure extending into the bottom of Fc pocket and resulting in strong ion and cation-pi interactions.
Moreover, a process of antigen-driven maturation was proven by somatically mutated VH residues on H2 and H3 CDR loops in the interfaces.
Taken together, these results suggested that high affinity IgG RFs can be generated in patients with Sjögren’s syndrome and may play an important role in the pathogenesis of this autoimmune disease.
American Psychological Association (APA)
Lee, Yu-Ching& Tsai, Keng-Chang& Leu, Sy-Jye& Wang, Duen-Jen& Liu, Chia-Yu& Yang, Yi-Yuan. 2013. Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1033014
Modern Language Association (MLA)
Lee, Yu-Ching…[et al.]. Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome. The Scientific World Journal No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1033014
American Medical Association (AMA)
Lee, Yu-Ching& Tsai, Keng-Chang& Leu, Sy-Jye& Wang, Duen-Jen& Liu, Chia-Yu& Yang, Yi-Yuan. Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sjögren’s Syndrome. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1033014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033014