![](/images/graphics-bg.png)
The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis
Joint Authors
Rogers, G. J.
Scheepers, Marius A.
Lecuona, Karin A.
Bunce, Catey
Corcoran, Craig
Michaelides, Michel
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Objective.
To assess the value of routine polymerase chain reaction (PCR) analysis on intraocular fluid from patients presenting with a first episode of suspected active infectious posterior uveitis in a population with a high prevalence of human immunodeficiency virus infection.
Design.
Retrospective, interventional case series.
Participants.
159 consecutive patients presenting at a tertiary care hospital over a five-year period.
Methods.
PCR analysis was performed for cytomegalovirus, varicella zoster virus, herpes simplex virus types 1 and 2, Toxoplasma gondii, and Mycobacterium tuberculosis.
Results.
PCR analysis confirmed the initial clinical diagnosis in 55 patients (35%) and altered the initial clinical diagnosis in 36 patients (23%).
The clinical diagnosis prior to PCR testing was nonspecific (uncertain) in 51 patients (32%), with PCR providing a definitive final diagnosis in 20 of these patients (39%); necrotizing herpetic retinopathy and ocular toxoplasmosis were particularly difficult to diagnose correctly without the use of PCR analysis.
Conclusion.
The clinical phenotype alone was unreliable in diagnosing the underlying infectious cause in a quarter of patients in this study.
Since the outcome of incorrectly treated infective uveitis can be blinding, PCR analysis of ocular fluids is recommended early in the disease even in resource poor settings.
American Psychological Association (APA)
Scheepers, Marius A.& Lecuona, Karin A.& Rogers, G. J.& Bunce, Catey& Corcoran, Craig& Michaelides, Michel. 2013. The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033048
Modern Language Association (MLA)
Scheepers, Marius A.…[et al.]. The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1033048
American Medical Association (AMA)
Scheepers, Marius A.& Lecuona, Karin A.& Rogers, G. J.& Bunce, Catey& Corcoran, Craig& Michaelides, Michel. The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033048
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033048